Neuroprotective Potential of Carbamide Forte in Lipopolysaccharide Induced Experimental Parkinson's Disease

Abstract

Background: Parkinson's disease is a second most common age-related neurodegenerative disorder characterized by the loss of dopaminergic neurons in the substantia nigra pars compacta region of the midbrain. Increased oxidative stress and inflammation with decreased activity of glutathione peroxidase, superoxide dismutase (SOD), and catalase has been observed in PD patients.Carbamide Forte Brain and Memory Support is blend of 9 herbal brain-enhancing ingredients to boost short term cognitive function andlong-term brain health andthus expected to be beneficial in cognition and movement disorder. Objective:The present study was designed to investigate Neuroprotective effect of Carbamide Forte Brain and Memory Support Supplement against Intranigral LPS induced Parkinsonism like symptoms in Sprague Dawley rats. Methods: Adult male/female Sprague Dawley rats were Intranigral injected with LPS at a dose of 0.2 mg/kg body weight into substantia nigra pars compacta with the help of stereotaxic apparatus. Carbamide Forte was administered per orally at 80 and 160 mg/kg body weight, 4 days after to LPS infusion. Results:Infusion of single Intranigral LPS produced significantly decreased in body weight, locomotor activity, motor coordination, antioxidant defense enzymes and significantly increased oxidative stress markers in the striatum. Chronic administration of carbamide forte (for21days) significantly attenuated LPSinduced movement disabilities in Narrow beam walk, Horizontal ladder beam walk test, OFT and also significantly reduce the oxidative-nitrosative stress and antioxidants enzymesas evidence by decrease in malondialdehyde and nitrite levels and restored the reduced glutathione level in rats& neuroprotective potential of CF with morphological changes seen in our histological assessment.The observed result of the present study is indicative of the therapeutic potential of Carbamide Forte in PD. Conclusion: It could be concluded that the observed neuroprotective effect of Carbamide Forte may be due toantioxidant and neuroprotective potential. Keywords:CarbamideForte,Intranigral, Dopamine, oxidative stress,Lipopolysaccharide(LPS),Parkinson’s disease.